Nepata cataria. (Catnip) is a perennial herb that belongs to the botanical family called lamiaceae (labiatae) and has many medicinal properties. This study focused on using cell and tissue culture system to increase produced secondary metabolites such as caffeic acid from cell suspension by effect of different concentrations of Plant Growth Regulators (PGRs). The concentration (2 mg L-1 2,4-D with 0.2 mg L-1 kinetin) showed the best result in growth of cell suspension, while the concentration (3 ml L-1 IAA with 0.3 ml L-1 BAP) showed normal result compared to other PGRs. No growth was noticed in cell suspension culture of the control treatment (free of PGRs). Findings of Centrifuge and Reduction of Tetrazolium salts confirmed these results. Result also showed significant difference in growth cell lines and production between cell lines and plants growth in vivo. The chemical analysis of High Performance Liquid Chromatograph (HPLC) showed that cell lines DKN1 produced the highest quantity of Caffeic acid while cell lines IKN 6 produced the lowest amount of Caffeic acid.

Key Words :Callus, High Performance Liquid Chromatograph (HPLC), Cell Suspension, Plant Growth Regulators (PGRs), 2,3,5-triphenyletetrazolium chloride )TTC), Nepeta cataria, Caffeic acid